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Image Search Results
Journal: Microorganisms
Article Title: Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis
doi: 10.3390/microorganisms9010087
Figure Lengend Snippet: RNAP levels during bacterial growth. Amounts of RNAP were detected by Western blotting from 5 μg of total protein per lane. Representative primary data are shown above the graph. The RNAP level from time point 1 was set as a 1. STA—stationary phase (indicated with the arrow). The experiment was conducted in two independents replicas. The points are averages, the error bars show the range. The dashed line shows a representative bacterial growth.
Article Snippet: After centrifugation (5 min, 4 °C) to remove cell debris, the amounts of proteins were measured with the Bradford protein assay and 5 μg was resolved by SDS-PAGE and analyzed by Western blotting, using mouse monoclonal antibodies against the β subunit of
Techniques: Western Blot